Optimization of pH, Temperature and Agitation Rate on Biodegradation of Lipids and Detergents in Food Wastewater by Bacillus sp N-09
نویسنده
چکیده
The purpose of this research was to determine the optimum conditions of pH, temperature and agitation rate for the biodegradation of lipids and detergent by Bacillus cereus N-09. A response surface method was used to analysis the data. The materials used in this research was the waste created by boiling instant noodles as a reference on the packaging and then diluted ten times and added a commercial detergent containing LAS as much as 1%. Three factors namely pH (6.0 to 8.0), temperature (28 32C) and agitation rate (100-140 rpm) were studied. Erlenmeyer flask filled with 100 ml medium and sterilized and then inoculated with Bacillus cereus N-09 age of 2 days. Incubation was carried out for 5 days. The results show that the optimal condition for pH was 6.00, temperature of 30C and agitation speed of 130rpm. In these conditions the degradation of lipids and detergents achieved respectively were 73.5% and 93.35%.
منابع مشابه
Carotenoid Production by Rhodotorula sp. on Fruit Waste Extract As a Sole Carbon Source and Optimization of Key Parameters
Fruit waste extract was selected as the sole substrate for carotenoid production and the effect of parameters like pH, temperature and agitation have been studied. A two-step simple sequential strategy was employed for the optimization of carotenoid production by Rhodotorula rubra. In the first step, one factor at a time was employed to evaluate the impact of pH on carotenoid production. Th...
متن کاملOptimization of factors affecting the application of Fenton process in sofa and detergent wastewater treatment
Introduction: Most commercial surfactants used for cleaning (as detergents (are part of xezenbiotic compounds. These compounds and products due to its toxic effects on aquatic organisms may interfere with hormonal system and consequently change the echo system. Since the biodegradation of those compounds are weak, ordinary methods are unable to break down conventional purification. Therefore, t...
متن کاملIsolation, Identification and Partial Optimization of Novel Xylanolytic Bacterial Isolates from Bhilai-Durg Region, Chhattisgarh, India
Background: Plant biomass and agricultural waste products disposal is a serious problem in agriculture based countries. These wastes, usually rich in xylan can be satisfactorily converted to industrially important and useful products by efficient biotechnological application of potent xylanase producing bacteria which generally have high temperature and pH optima....
متن کاملStudy on Activity and Stability of Proteases from Bacillus Sp. Produced by Submerged Fermentation
Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...
متن کاملNovel Bacterial Strains Pseudomonas sp. and Bacillus sp. Isolated from Petroleum Oil Contaminated Soils for Degradation of Flourene and Phenanthrene
Flourene and phenanthrene are organic compounds with high hydrophobicity and toxicity. Being recalcitrant in nature they are accumulating in the environment at an alarming concentration, posing serious threat to living beings. Thus in the present study, microorganisms were screened for their ability to degrade these contaminants at high concentrations in least period of time. Two out of fifteen...
متن کامل